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Titers were calculated as the reciprocal serum dilution at 50% of the maximum optical density (OD50).
The inhibitory dilution at 50% neutralization (ID50 titers) was determined by non-linear regression by the method of least squares.
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Optimum operating conditions was found to be; amount of dilution at 30%, initial pH of 6, applied current of 14 mandm2 and electrolyte dose of 1075 mg/l and it shows the 93% of COD removal rate and 95% of COD removal with electrical energy consumption value of 3.48 KW h/l.
In response to low dilution rate at 50% oxygen saturation, 128 genes involved in energy metabolism were upregulated and 62 downregulated.
FcERα titre was determined by plotting the absorbance of titrated samples, and reading off the dilution factor at 50% inhibition, compared to the standard.
The 50% endpoint dilution of each serum, corresponding to the dilution at which 50% of the wells were completely protected from infection, was determined according to the Reed Muench method.
The inverse of the dilution at which 50% of the wells showed cytopathic effect was recorded as the 50% tissue culture infectious dose (TCID50).
A typical classical pathway (CH50) test was done using 100 μl of goat serum serial 2-fold dilutions (starting at 50% serum, serially diluting 10 times) in DGVB++, mixed with 100 μl of 10/ml EA cells in DGVB++.
Neutralizing antibody titers were calculated as the highest dilution at which 50% of the cells stained blue by visual inspection.
NAb titers were defined as the reciprocal of the highest serum dilution at which 50% of wells were protected.
The highest serum dilution at which 50% agglutination was observed was marked as the end point for titre.
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